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In this study, we combined correlative light and electron microscopy (CLEM), 3D electron tomography (ET), cryo-EM, and all-atom molecular dynamics simulations, along with cell biology and genetic approaches, to investigate plant retrograde transport in the model plant Arabidopsis thaliana , with the following major findings: (1) The CLEM, 3D ET, and immunogold transmission electron microscopy (TEM) analyses revealed distinct spherical vesicles (~3050 nm in diameter) labeled by retromer markers and VSR proteins in Arabidopsis root cells, with nascent vesicles budding from MVBs in Arabidopsis root cells

Fluorescence was read at Ex/Em = 535/587nm on BioTek HT Synergy Plate Reader

: Classification and adaptive behavior prediction of children with autism spectrum disorder based upon multivariate data analysis of markers of oxidative stress and DNA methylation

Reduction of the CD4 CSD is mediated by Trx, Grx1, and PDI in vitro (Gallina et al., 2002